Detection Kit for β2-microglobulin (β2-MG)
【Product Name】Detection Kit for β2-microglobulin (β2-MG) (enhanced chemiluminescence immunoassay)
【Registration certificate number】 京械注准20172401083
This kit is mainly used for quantitative determination of β2-microglobulin (β2-MG) in human serum and urine in vitro.
β2-microglobulin (β2-MG) is a single-chain polypeptide consisting of 100 amino acid residues with a molecular weight of 11.8 kD and is mainly synthesized by nucleated cells such as lymphocytes. It determine the β2-MG content of serum or urine, its result and the results of urine albumin and IgG is a synergistic assessment of renal function, which also an important basis of identification of kidney injury sites, assessment of diabetes, hypertension and drug damage to the renal function. It also provides a reference for the clinical diagnosis of some tumors and cancers.
This kit uses competitive approach. Microplate was coated withβ2-MG antibody, enzyme-labeled antigen competes with unlabeled antigen (antigen of test sample) for coating a limited number of wells binding site of the specific antibody in the microplate and combines into antigen-Antibody complex, the number of unlabeled antigen (antigen of test sample ) affects the number of antigen-antibody complex, the more antigens in the sample to be tested, the less labeled antigen-antibody complex formed. Unbound labeled antigen is removed after the immune reaction, it cancan produce optical signals by adding chemiluminescent substrate, the optical signal will decrease with the increase of antigen in the sample to be tested. According to the relative luminescence intensity of β2-MG series and its concentration, the dose-response curve can be drawn by log-logit mathematical model or other suitable mathematical model fitting and the content of β2-MG in the tested sample can be calculated from this curve by the relative luminescence intensity.
Clinical laboratory, physical examination center.