Detection Kit for folic acid
【Product Name】Detection Kit for folic acid (enhanced chemiluminescence immunoassay)
【Registration certificate number】 京械注准20172401078
【Packing】48 tests/box,96 tests/box.
【Storage conditions and validity】Storage at 2 ~ 8℃,validity for 6 months.
This kit is mainly used for quantitative determination of folic acid in human serum in vitro.
The deficiency of can lead to nutritional and giant cell anemia. Folic acid deficiency can be seen in chronic alcoholism, drug addicts, the elderly and the poor. In addition, low levels of serum folate during pregnancy can lead to fetal neural tube defects. Because the deficiency of vitamin B12 can also cause megaloblastic anemia, so it is necessary to detect content of vitamin B12 and folic acid for diagnosing the cause of anemia.
This kit uses competitive approach. The reagents used in the kit are folic acid standard, horseradish peroxidase labeled folic acid (HRP-folic acid), microplate coated with folic acid antibody, chemiluminescent substrate (with enhancer), enzyme-labeled antigen competes with unlabeled antigen (antigen of test sample) for coating a limited number of wells binding site of the specific antibody in the microplate and combines into antigen-Antibody complex, the number of unlabeled antigen (antigen of test sample ) affects the number of antigen-antibody complex, the more antigens in the sample to be tested, the less labeled antigen-antibody complex formed. Unbound labeled antigen is removed after the immune reaction, it cancan produce optical signals by adding chemiluminescent substrate, the optical signal will decrease with the increase of antigen in the sample to be tested. According to the relative luminescence intensity of folate series and its concentration, the dose-response curve can be drawn by log-logit mathematical model or other suitable mathematical model fitting and the content of folate in the tested sample can be calculated from this curve by the relative luminescence intensity.
1. The clinical manifestations of folic acid and vitamin B12 deficiency are basically similar, it all can cause megaloblastic anemia, leucopenia, thrombocytopenia, and gastrointestinal symptoms such as loss of appetite, bloating, diarrhea and glossitis, glossitis is the most prominent symptom, tongue is red, tongue atrophy, smooth surface, commonly known as "beef tongue", it is also accompanied with pain. Pediatric and elderly patients often have psychiatric symptoms such as no desire, drowsiness or confusion.
2. Lack of folic acid can cause emotional changes, it can disappear by replenishing folic acid. Pregnant women lack of folic acid, can cause pre-eclampsia, the incidence of placental exfoliation increased, pregnant women with megaloblastic anemia prone to intrauterine growth retardation, low birth weight of premature birth and neonatal. Lack of folic acid at early pregnancy will easily lead to fetal neural tube defects (such as spina bifida, anencephaly, etc.). Folic acid deficiency can cause hyperhomocysteinemia, thereby increasing the risk of cardiovascular disease. Small intestine disease can interfere with the absorption of food folic acid and the process of re-enterohepatic circulation, so folic acid deficiency is a common complication of intestinal diseases.
Clinical laboratory, physical examination center.